Ion-Pairing with Triethylammonium Acetate Improves Solid-Phase Extraction of ADP-Ribosylated Peptides
نویسندگان
چکیده
منابع مشابه
Identification of ADP-ribosylated peptides and ADP-ribose acceptor sites.
ADP-ribosylation is a post-translational modification of proteins that comprises the transfer of the ADP-ribose moiety from NAD+ to specific amino acid residues on substrate proteins or to ADP-ribose itself. It is catalyzed by ADP-ribosyltransferases, a family of currently 22 human proteins that all possess an ADP-ribosyltransferase catalytic domain. ADP-ribosylation is a reversible modificatio...
متن کاملADPriboDB: The database of ADP-ribosylated proteins
ADP-ribosylation refers to the addition of one or more ADP-ribose units onto proteins post-translationally. This protein modification is often added by ADP-ribosyltransferases, commonly known as PARPs, but it can also be added by other enzymes, including sirtuins or bacterial toxins. While past literature has utilized a variety of methods to identify ADP-ribosylated proteins, recent proteomics ...
متن کاملIdentification and analysis of ADP-ribosylated proteins.
The analysis of ADP-ribosylated proteins is a challenging task, on the one hand because of the diversity of the target proteins and the modification sites, on the other hand because of the particular problems posed by the analysis of ADP-ribosylated peptides. ADP-ribosylated proteins can be detected in in vitro experiments after the incorporation of radioactively labeled or chemically modified ...
متن کاملSolid-phase Extraction with Discs
Berrueta LA, Gallo B and Vicente F (1995) A review of solid-phase extraction: basic principles and new developments. Chromatographia 40(7/8): 474}483. Guzman NA, Park SS, Schaufelberger D et al. (1997) Review: new approaches in clinical chemistry: on-line analyte concentration and microreaction capillary electrophoresis for the determination of drugs, metabolic intermediates and biopolymers in ...
متن کاملSilver ion chromatography using solid-phase extraction columns packed with a bonded-sulfonic acid phase.
Commercial solid-phase extraction columns packed with a stationary phase with bonded benzenesulfonic acid groups are readily converted to the silver ion form and can then be used for silver ion chromatography of lipids. To illustrate the utility of such procedures, methyl ester derivatives of fatty acids with zero to six double bonds were separated from each other by a simple stepwise elution s...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2019
ISSN: 1535-3893,1535-3907
DOI: 10.1021/acs.jproteome.9b00696